1. Investigation of the potential for natural transporters to
be used for oral delivery of peptides and proteins.
Demonstrated that Vitamin B12 could
transport proteins, polymers and nanoparticles from the gut into the circulation
Technology developed for oral delivery
of protein phamaceuticals
USP 6,262,253 ; USP 6,221,397;
2. Investigation of the potential to use vitamins as targeting agents for cancer
VB12, folate and biotin compared for
their ability to image various tumour cell lines.
Aggressive tumours found to over-express
receptors involved in the uptake of folate, or vitamin B12 and
Vitamin-targeted [dug-polymer] complexes
showed enhanced killing of tumours
Variable results found with different
tumours with cytotoxins such as methotrexate, daunomycin, and platinum drugs
3. Development of transdermal delivery system for peptides and
Specific water-in-oil microemulsions
found to be active in transdermal transport of peptides and proteins
Topical anti-inflammatory developed with
tetrahydrocurcumin and adenosylcobalamin
Topical anti-inflammatory activity shown
with Enbrel, Humira and Remicade
4. Development of novel nanolattice system for oral delivery
Demonstration of potential for zinc chelates to act as X-linkers in the
preparation of nanolattices within a microemulsion.
Folate, biotin, riboflavin and vitamin
B12 found to be active as oral targeting agents
Oral delivery of insulin shown.
Oral delivery of anti-TNF molecules
5. Literature Review of
Potential Carriers for Oral Protein Delivery
10,000 word literature review on the
state of the art and potential of oral transporters. >160 Citations
Several novel transporters identified.
Review to Examine the Effect of Tobacco Additives on the Bio-availability of
10,000 word literature review
Contract Number 98-99 HAU/C7 to Australian Government. >90
7. Identification of the functional role of serum IgA
Examined the potential for serum IgA to
act as a blocking antibody
Demonstrated the major functional role of serum IgA is to act as a blocking
antibody for complement-mediated
and Immediate Type
8. Isolation of the major protein antigens of Group B
Purified the Ibc protein antigens.
Raised monoclonal antibodies to both
Demonstrated that the major protein
antigen was an IgA binding protein
Developed an ELISA assay for N.
gonorrhoea based on detection of the IgA1 protease.
9. Development of a diagnostic ELISA kit for the detection of
salmonella in food
Serotyped Flagella antigens from major
antigenic groups so salmonella
Developed large scale purification
system for LPS-free protein antigens and standards
Prepared affinity columns to generate
Identified cross-reactive organisms
Isolated antigens and prepared affinity
column for removal of cross-reactive antibodies.
Developed diagnostic kit for detection
of Salmonella in food.
Establishment of TECRA International
10.Optimization of adjuvant formulations for veterinary
Compared various adjuvants for effective
use in sheep, cattle, dogs and cats.
Identified powerful T cell epitopes in bacterial outer
Identified a specific class of universal
T cell epitopes in a number of self-adjuvanting protein antigens
Generation of LHRH-TraT fusion proteins
for generation of immune-castration in dogs
of a protective vaccine for neonatal scours
Developed a purification system for E.
coli pili and for LTB.
Identified a new class of oral vaccine carriers, LTB-fusion proteins, lectin-like
carriers for oral protein delivery.